pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants.
Identifieur interne : 000381 ( Main/Exploration ); précédent : 000380; suivant : 000382pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants.
Auteurs : Dipak Kumar Sahoo [États-Unis] ; Nrisingha Dey [Inde] ; Indu Bhushan Maiti [États-Unis]Source :
- PloS one [ 1932-6203 ] ; 2014.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), ADN des plantes (génétique), ARN messager (génétique), Agrobacterium tumefaciens (croissance et développement), Agrobacterium tumefaciens (génétique), Agrobacterium tumefaciens (métabolisme), Arabidopsis (croissance et développement), Arabidopsis (génétique), Arabidopsis (métabolisme), Dosage biologique (MeSH), Escherichia coli (croissance et développement), Escherichia coli (génétique), Escherichia coli (métabolisme), Glucuronidase (génétique), Glucuronidase (métabolisme), Plasmides (génétique), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), RT-PCR (MeSH), Réaction de polymérisation en chaine en temps réel (MeSH), Tabac (croissance et développement), Tabac (génétique), Tabac (métabolisme), Transformation génétique (MeSH), Transgènes (génétique), Vecteurs génétiques (MeSH), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- croissance et développement : Agrobacterium tumefaciens, Arabidopsis, Escherichia coli, Tabac, Végétaux génétiquement modifiés.
- génétique : ADN bactérien, ADN des plantes, ARN messager, Agrobacterium tumefaciens, Arabidopsis, Escherichia coli, Glucuronidase, Plasmides, Protéines à fluorescence verte, Tabac, Transgènes, Végétaux génétiquement modifiés.
- métabolisme : Agrobacterium tumefaciens, Arabidopsis, Escherichia coli, Glucuronidase, Protéines à fluorescence verte, Tabac, Végétaux génétiquement modifiés.
- Dosage biologique, RT-PCR, Réaction de polymérisation en chaine en temps réel, Transformation génétique, Vecteurs génétiques.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (growth & development), Agrobacterium tumefaciens (metabolism), Arabidopsis (genetics), Arabidopsis (growth & development), Arabidopsis (metabolism), Biological Assay (MeSH), DNA, Bacterial (genetics), DNA, Plant (genetics), Escherichia coli (genetics), Escherichia coli (growth & development), Escherichia coli (metabolism), Genetic Vectors (MeSH), Glucuronidase (genetics), Glucuronidase (metabolism), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Plasmids (genetics), RNA, Messenger (genetics), Real-Time Polymerase Chain Reaction (MeSH), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Tobacco (genetics), Tobacco (growth & development), Tobacco (metabolism), Transformation, Genetic (MeSH), Transgenes (genetics).
- MESH :
- chemical , genetics : DNA, Bacterial, DNA, Plant, Glucuronidase, Green Fluorescent Proteins, RNA, Messenger.
- genetics : Agrobacterium tumefaciens, Arabidopsis, Escherichia coli, Plants, Genetically Modified, Plasmids, Tobacco, Transgenes.
- growth & development : Agrobacterium tumefaciens, Arabidopsis, Escherichia coli, Plants, Genetically Modified, Tobacco.
- metabolism : Agrobacterium tumefaciens, Arabidopsis, Escherichia coli, Glucuronidase, Green Fluorescent Proteins, Plants, Genetically Modified, Tobacco.
- Biological Assay, Genetic Vectors, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Transformation, Genetic.
Abstract
We have constructed a small and highly efficient binary Ti vector pSiM24 for plant transformation with maximum efficacy. In the pSiM24 vector, the size of the backbone of the early binary vector pKYLXM24 (GenBank Accession No. HM036220; a derivative of pKYLX71) was reduced from 12.8 kb to 7.1 kb. The binary vector pSiM24 is composed of the following genetic elements: left and right T-DNA borders, a modified full-length transcript promoter (M24) of Mirabilis mosaic virus with duplicated enhancer domains, three multiple cloning sites, a 3'rbcsE9 terminator, replication functions for Escherichia coli (ColE1) and Agrobacterium tumefaciens (pRK2-OriV) and the replicase trfA gene, selectable marker genes for kanamycin resistance (nptII) and ampicillin resistance (bla). The pSiM24 plasmid offers a wide selection of cloning sites, high copy numbers in E. coli and a high cloning capacity for easily manipulating different genetic elements. It has been fully tested in transferring transgenes such as green fluorescent protein (GFP) and β-glucuronidase (GUS) both transiently (agro-infiltration, protoplast electroporation and biolistic) and stably in plant systems (Arabidopsis and tobacco) using both agrobacterium-mediated transformation and biolistic procedures. Not only reporter genes, several other introduced genes were also effectively expressed using pSiM24 expression vector. Hence, the pSiM24 vector would be useful for various plant biotechnological applications. In addition, the pSiM24 plasmid can act as a platform for other applications, such as gene expression studies and different promoter expressional analyses.
DOI: 10.1371/journal.pone.0098988
PubMed: 24897541
PubMed Central: PMC4045853
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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(MeSH)</term><term>DNA, Bacterial (genetics)</term><term>DNA, Plant (genetics)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (growth & development)</term><term>Escherichia coli (metabolism)</term><term>Genetic Vectors (MeSH)</term><term>Glucuronidase (genetics)</term><term>Glucuronidase (metabolism)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (metabolism)</term><term>Plasmids (genetics)</term><term>RNA, Messenger (genetics)</term><term>Real-Time Polymerase Chain Reaction (MeSH)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Tobacco (genetics)</term><term>Tobacco (growth & development)</term><term>Tobacco (metabolism)</term><term>Transformation, Genetic (MeSH)</term><term>Transgenes (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>ADN des plantes (génétique)</term><term>ARN messager (génétique)</term><term>Agrobacterium tumefaciens (croissance et développement)</term><term>Agrobacterium tumefaciens (génétique)</term><term>Agrobacterium tumefaciens (métabolisme)</term><term>Arabidopsis (croissance et développement)</term><term>Arabidopsis (génétique)</term><term>Arabidopsis (métabolisme)</term><term>Dosage biologique (MeSH)</term><term>Escherichia coli (croissance et développement)</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Glucuronidase (génétique)</term><term>Glucuronidase (métabolisme)</term><term>Plasmides (génétique)</term><term>Protéines à fluorescence verte (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>RT-PCR (MeSH)</term><term>Réaction de polymérisation en chaine en temps réel (MeSH)</term><term>Tabac (croissance et développement)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Transformation génétique (MeSH)</term><term>Transgènes (génétique)</term><term>Vecteurs génétiques (MeSH)</term><term>Végétaux génétiquement modifiés (croissance et développement)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Plant</term><term>Glucuronidase</term><term>Green Fluorescent Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Arabidopsis</term><term>Escherichia coli</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium 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xml:lang="en">We have constructed a small and highly efficient binary Ti vector pSiM24 for plant transformation with maximum efficacy. In the pSiM24 vector, the size of the backbone of the early binary vector pKYLXM24 (GenBank Accession No. HM036220; a derivative of pKYLX71) was reduced from 12.8 kb to 7.1 kb. The binary vector pSiM24 is composed of the following genetic elements: left and right T-DNA borders, a modified full-length transcript promoter (M24) of Mirabilis mosaic virus with duplicated enhancer domains, three multiple cloning sites, a 3'rbcsE9 terminator, replication functions for Escherichia coli (ColE1) and Agrobacterium tumefaciens (pRK2-OriV) and the replicase trfA gene, selectable marker genes for kanamycin resistance (nptII) and ampicillin resistance (bla). The pSiM24 plasmid offers a wide selection of cloning sites, high copy numbers in E. coli and a high cloning capacity for easily manipulating different genetic elements. It has been fully tested in transferring transgenes such as green fluorescent protein (GFP) and β-glucuronidase (GUS) both transiently (agro-infiltration, protoplast electroporation and biolistic) and stably in plant systems (Arabidopsis and tobacco) using both agrobacterium-mediated transformation and biolistic procedures. Not only reporter genes, several other introduced genes were also effectively expressed using pSiM24 expression vector. Hence, the pSiM24 vector would be useful for various plant biotechnological applications. In addition, the pSiM24 plasmid can act as a platform for other applications, such as gene expression studies and different promoter expressional analyses. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24897541</PMID><DateCompleted><Year>2015</Year><Month>10</Month><Day>22</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>6</Issue><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants.</ArticleTitle><Pagination><MedlinePgn>e98988</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0098988</ELocationID><Abstract><AbstractText>We have constructed a small and highly efficient binary Ti vector pSiM24 for plant transformation with maximum efficacy. In the pSiM24 vector, the size of the backbone of the early binary vector pKYLXM24 (GenBank Accession No. HM036220; a derivative of pKYLX71) was reduced from 12.8 kb to 7.1 kb. The binary vector pSiM24 is composed of the following genetic elements: left and right T-DNA borders, a modified full-length transcript promoter (M24) of Mirabilis mosaic virus with duplicated enhancer domains, three multiple cloning sites, a 3'rbcsE9 terminator, replication functions for Escherichia coli (ColE1) and Agrobacterium tumefaciens (pRK2-OriV) and the replicase trfA gene, selectable marker genes for kanamycin resistance (nptII) and ampicillin resistance (bla). The pSiM24 plasmid offers a wide selection of cloning sites, high copy numbers in E. coli and a high cloning capacity for easily manipulating different genetic elements. It has been fully tested in transferring transgenes such as green fluorescent protein (GFP) and β-glucuronidase (GUS) both transiently (agro-infiltration, protoplast electroporation and biolistic) and stably in plant systems (Arabidopsis and tobacco) using both agrobacterium-mediated transformation and biolistic procedures. Not only reporter genes, several other introduced genes were also effectively expressed using pSiM24 expression vector. Hence, the pSiM24 vector would be useful for various plant biotechnological applications. In addition, the pSiM24 plasmid can act as a platform for other applications, such as gene expression studies and different promoter expressional analyses. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sahoo</LastName><ForeName>Dipak Kumar</ForeName><Initials>DK</Initials><AffiliationInfo><Affiliation>KTRDC, College of Agriculture, Food and Environment, University of Kentucky, Lexington, Kentucky, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dey</LastName><ForeName>Nrisingha</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Gene Function and Regulation, Institute of Life Sciences, Bhubaneswar, Odisha, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maiti</LastName><ForeName>Indu Bhushan</ForeName><Initials>IB</Initials><AffiliationInfo><Affiliation>KTRDC, College of Agriculture, Food and Environment, University of Kentucky, Lexington, Kentucky, United States of America.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>06</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C036483">T-DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001681" MajorTopicYN="Y">Biological Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="Y">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName><QualifierName UI="Q000235" 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